polyclonal rabbit anti kiss1  (Bioss)

Journal: Journal of Ovarian Research

Article Title: Bone marrow mesenchymal stem cells expressing Neat-1, Hotair-1, miR-21, miR-644, and miR-144 subsided cyclophosphamide-induced ovarian insufficiency by remodeling the IGF-1–kisspeptin system, ovarian apoptosis, and angiogenesis

doi: 10.1186/s13048-024-01498-x

Figure Lengend Snippet: Effect of BM-MSCs transplantation on the expression of ovarian Kisspeptin system of the POI rats ( A – I ). Representative images from the anti-Kiss1 protein immunostained rat ovarian section of the CON ( A , B ), POI ( C , D ), and POI + BM-MSCs groups ( E , F ) lower and higher magnification, respectively, showing cytoplasmic immune expression of germinal epithelium (GE), interstitial stromal cells (IS), and granulosa lutein cells (GL) as intense, faint, and moderate immunoreactivity in CON, POI, and POI + BM-MSCs groups, respectively. Scale bars A , C , E = 200 μm; B , D , F = 50 μm, G . Relative expression of ovarian mRNA Kiss-r1, H. Relative expression of ovarian mRNA Kiss-1, and I . Area % of ovarian Kiss-1 positive immunostaining. The values represent the average of 6 to 8 rats in each group ± SEM * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Immunohistochemistry for VEGF and Kiss-1 was done using a primary polyclonal rabbit anti-VEGF-A (ABclonal, Cat. No. # A0280) and a primary rabbit polyclonal anti-kisspeptin antibody (Bioss, Cat. No. # bs-0749R) and their related secondary antibodies.

Techniques: Transplantation Assay, Expressing, Immunostaining

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Primary and secondary antisera used for Western blot, IFL, and IHC.

Article Snippet: Kiss1R rabbit polyclonal (BS-2501R, Bioss Antibodies, USA) , 1:200 (IF) 1:50 (IHC).

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of Kiss1R, Kiss1, Cyp19, GnRH , and miR expression in mediobasal hypothalamus homogenates. Representative Western blots ( n = 4) for Kiss1R, Kiss1, and Cyp19 (A) and normalization of Kiss1R (B) , Kiss1 (C) , and Cyp19 signals (D) carried out against α-tubulin. Quantitative expression ( n = 6) of GnRH (E) and miR-132-3p , Mir-145-5p , let-7a-5p , and let-7b-5p (F–I) carried out by qPCR. Data are expressed as protein OD/tubulin OD ± standard deviation or normalized fold expression (n.f.e.) ± SEM against the housekeeping genes HPRT / β-actin / U6 and the control group used as reference sample. C, control group (placebo injected animals); KP, animals injected with Kp10; AEA, animals injected with AEA; SR + AEA (SA), animals first received SR141716A and 30 min later received AEA treatment. ** p < 0.01, * p < 0.05 vs. the control group.

Article Snippet: Kiss1R rabbit polyclonal (BS-2501R, Bioss Antibodies, USA) , 1:200 (IF) 1:50 (IHC).

Techniques: Expressing, Western Blot, Standard Deviation, Injection

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Expression and distribution of Kiss1R and Kiss1 proteins in the hypothalamic arcuate nucleus (ARC). Representative images ( n = 4 for each animal group). Nissl staining showing ARC localization at ×20 magnification (A) ; IHC of Kiss1R expression modulation in ARC, ×20 magnification (B) ; IF analysis of the Kiss1 expression in ARC, ×40 magnification (C) . C, control; KP, animals injected with Kp10; AEA, animals injected with AEA; SR + AEA, animals first received SR141716A and 30 min later received AEA treatment. 3V, third ventricle. ** p < 0.01; * p < 0.05.

Article Snippet: Kiss1R rabbit polyclonal (BS-2501R, Bioss Antibodies, USA) , 1:200 (IF) 1:50 (IHC).

Techniques: Expressing, Staining, Injection

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of the KS by KP (A–C) and AEA (D–F) in rat testis. Representative Western blots for Kiss1R and Kiss1 (A, D) and normalization of Kiss1R (B, E) and Kiss1 signals (C, F) carried out against α-tubulin. N = 6 different animals in KP treatments and n = 4 in AEA treatments; data are expressed as protein OD/tubulin OD ± standard deviation. C, control group (placebo-injected animals); KP, animals injected with Kp10; AEA, animals injected with AEA; SR + AEA, animals first received SR141716A and 30 min later received AEA treatment. * p < 0.05.

Article Snippet: Kiss1R rabbit polyclonal (BS-2501R, Bioss Antibodies, USA) , 1:200 (IF) 1:50 (IHC).

Techniques: Western Blot, Standard Deviation, Injection

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Distribution of Kiss1 and Kiss1R in rat testis treated with KP and AEA. Double immunofluorescence of Kiss1 (red) and Kiss1R (green) expression in control (C), kisspeptin (KP), anandamide (AEA), and SR141716A (SR + AEA)-treated animals ( n = 4 for each group). The last column shows the representative images of DAPI staining on the same animals. Magnification = C ×40; KP, AEA, and SR+ AEA, ×63. * Elongated spermatids, ↑ spermatocytes, ^ spermatogonia, ° interstitium.

Article Snippet: Kiss1R rabbit polyclonal (BS-2501R, Bioss Antibodies, USA) , 1:200 (IF) 1:50 (IHC).

Techniques: Immunofluorescence, Expressing, Staining